Sounds like your "tic", under load, in 6th gear is detonation; a down shift at 3200 would most likely taken you out of the "tic" range. The CCP (cold cranking compression) generated by the combination of 103" with SE 255 cams with .045" stock head gasket is just under 200psi. Depending on ambient temps, the "load" quality of fuel and tune might have created the "perfect storm" for detonation to present. The fact that the condition repeated on the two up, loaded with camping gear ride supports the potential for detonation. The fact that the condition did not replicate with the saved tune (not knowing what the saved tune is) also supports the detonation theory.
The noise is described as a "tic" which is not the typical description for detonantion which is usuall descrbed as "marbles bouncing on the top of the pistons". The term "tic" is usually associated with the valve train which can be caused by a lifter not holding its prime. However, if it was a lifter ticking, the noise should have presented regardless of which tune was running. Are you running adjustable or OEM pushrods?
Are you running 93/97 octane fuel? Was the new 103" motor configuration dyno tuned and by who? Was the "saved" tune the OEM tune? I know the tic presented at 3200rpms but how fast were you going when the tic presented. Suggest that you reload the tune that presented the tic and when the tic presents, down shift to 5th and see what happens.
Of course, you should be able to cruise at 3000 to 3200rpms at 70mph without having to worry about detonation which will kill an air cooled vtwin if not managed. I have had personal experience with this same situation. I ran a cam in a 95" motor with an early intake close which generated CCP just under 200psi. I live/ride in southe Texas; hot summers. The motor ran fine in the cooler months of the year but started to detonate in July/August when ambient temps approached 100*. We tried to pull timing but when we pulled enough to elimiate the detonation, the bike was a dog; a cam change and retune was the fix to return the performance to where it was before.
I see three ways going forward.
1. If you are happy with the "saved' tune, i.e., no detonation, you can run that tune
2. Get the bike back on the dyno with a competent tuner and see if he can tune the detonation out.
3. Worst case; if a retune doesn't maintain the same level of performance and allow you to cruise at 70mph in sixth gear without the detonation, then a cam change and retune may be the only solution; you have to eliminate the detonation. or you will be rebuilding again.
It is hard to diagnose problems like this from a post, If I could ride the bike, I could be sure of what I have posted which is JMHO. So apologies for the winded post but the "devil is in the details" and I focus on the details. If any of the above information is not an accurate understanding of your situation and question, feel free to correct; I just want to help out.