Did I miss it ? I didn't see any reply about the dual fire of the old Harleys. That caused more of a distinctive difference in the sound than anything else that I've been able to tell.
The never ones only fire separately in each cylinder at a time, whereas the older ones fired in both cylinders at the same time. One cylinder was actually ready to receive the fire at the top of it's compression stroke. The other was either on it's intake or exhaust stroke depending on which cylinder in the cycle.
This gave a very distinctive potato potato sound that is different than the newer models.